Panels

DOT Drug Test (5-Panel)

DOT-regulated employers must test to the federal 49 CFR Part 40 standard — a specific 5-panel with strict chain of custody and Medical Review Officer (MRO) review. LabX runs DOT-compliant collection and confirmation so your drivers and safety-sensitive employees stay compliant.

What the DOT 5-panel covers

The DOT panel is federally defined: Marijuana (THC), Cocaine, Amphetamines/Methamphetamine, Opioids (including codeine, morphine, hydrocodone, hydromorphone, oxycodone, oxymorphone), and Phencyclidine (PCP). Since 2018 the opioid group includes the semi-synthetics.

Chain of custody & MRO review

Every DOT test uses a Federal Custody and Control Form, LC-MS/MS confirmation of non-negatives, and independent MRO verification before a result is released. This is what makes a DOT result stand up to a challenge.

Non-DOT vs. DOT

If you are not federally regulated, a 10-panel or 14-panel gives you broader coverage and more flexibility. Read DOT vs non-DOT drug testing for the full comparison.

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Common questions

What drugs are on the DOT panel?
Marijuana, cocaine, amphetamines/methamphetamine, opioids (including semi-synthetics), and PCP — as defined by 49 CFR Part 40.
Do DOT tests require an MRO?
Yes. A Medical Review Officer must review and verify every DOT drug test result before it is reported.
Can I use a DOT test for non-DOT employees?
You can, but non-DOT roles are usually better served by a broader 10- or 14-panel that you control.
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